Research Article

Potential Clinical Significance of Cytotoxicity Evaluation of Biodegradable Mg-6Zn Alloy on IEC-6, CT26 and A7r5 Cell Lines

Author: Leiming Zhu

The recent using of Mg-based Mg-6Zn alloys for biodegradable implants was not supported with sufficient toxicity evaluation data. This work presented a thorough cytotoxicity evaluation of Mg-6Zn alloy for biodegradable implants. Three cell lines (IEC-6, CT26 and A7r5 cells) were used to evaluate the cytotoxicity of Mg-6Zn alloy on intestinal wall cells in vitro. Cell viability and proliferation capability were assessed by MTT assay. Cell proliferation was investigated by flow cytometric analysis. Three types of cell lines were exposed to Mg-6Zn alloy extracts for 24h, 48h and 72 hours for subsequent research assay, the cytotoxicity assay of Mg-6Zn alloy extracts at different concentrations and the effect on cells factors expression levels were studied, and the biodegradation capability of Mg-6Zn alloy was also studied. The results indicated that, with the increasing of the concentrations of Mg-6Zn alloy extract, the impact of Mg-6Zn alloy on the viability and proliferation capability of three types of cell lines was not obvious, but when the culture time was prolonged, the Mg alloy extract as an obvious inhibitory effect on colon cancer cell CT26 growth (P<0.05). Although Mg-6Zn alloy extracts could have slight effect on the cell cycle of CT26 cell, there was no significant difference when compared with IEC-6. Mg alloys was subjected to slight corrosion after 3 weeks of immersion in the intestinal juice, and severe dramatic corrosion was observed at fourth weeks. In conclusion, Mg-6Zn alloy can be suggested as a suitable candidate of biodegradable implants to be used in biomedical applications